Study on the Converted Total Least Squares method and its application in coordinate transformation

This thesis gives a brief introduction to Total Least Squares (TLS) comparing with the classical LS, and its common solutions by singular value decomposition (SVD) approaches and the iteration, also following with the advantages and disadvantages of both methods. One method named Converted Total Least Squares (CTLS) dealing with the errors-in-variables (EIV) model can solve the problems of both. The basic idea of it is to take the stochastic design matrix elements as virtual observations, and the TLS problem can be transformed into a LS problem. The significance of CTLS lies not merely in attaining the optimal estimation of parameters and more importantly in completing the theory of TLS with classical LS. As a comparison, another estimation method based on Partial-EIV model will also be presented, which can deal with the TLS problems with iterative algorithm. The coordinate transformation parameter estimation formula of both algorithms are derived. By specifying the accuracy assessment formulas of CTLS, this thesis identifies rigorously the degree of freedom of the EIV model in theory and solves the bottleneck problem of TLS that restricts the application and development of TLS

Converted Total Least Squares method and Gauss-Helmert model with applications to transformation among ITRF realizations

This thesis is an extension and improvement of the theory and applications of Converted Total Least Squares method (CTLS). Converted Total Least Squares (CTLS) dealing with the errors-in-variables (EIV) model take the stochastic design matrix elements as virtual observations, and the TLS problem can be transformed into a LS problem. In the coordinate transformation, the transformation model is always used after centering like it is published in most papers. This thesis directly uses the transformation model to generate a new design matrix with CTLS method. The result will present the consistency of the transformation model with and without centering in coordinates transformation. Then the 3D Helmert-transformation in Gauss-Helmert and Gauss-Markoff model is introduced (Koch 2002). The study is to find that, the connections between CTLS and the Gauss-Helmert model. To prove their similarity is a strong support for the theory of the CTLS method. After that, this thesis gives a brief introduction to the International Terrestrial Reference System (ITRF). The CTLS has been proved itself with coordinate transformation in Baden-Württemberg with equal weight and large scale. The new application with more parameters and smaller scale together with the weight information in ITRF is presented. The comparison and accuracy assessment of the published parameters and the parameters estimated by CTLS are discussed in detail with the applications

Prognostic significance of snail expression in hilar cholangiocarcinoma

Many patients with hilar cholangiocarcinoma (HC) have a poor prognosis. Snail, a transcription factor and E-cadherin repressor, is a novel prognostic factor in many cancers. The aim of this study was to evaluate the relationship between snail and E-cadherin protein expression and the prognostic significance of snail expression in HC. We examined the protein expression of snail and E-cadherin in HC tissues from 47 patients (22 males and 25 females, mean age 61.2 years) using immunohistochemistry and RT-PCR. Proliferation rate was also evaluated in the same cases by the MIB1 index. High, low and negative snail protein expression was recorded in 18 (38%), 17 (36%), and 12 (26%) cases, respectively, and 40.4% (19/47) cases showed reduced E-cadherin protein expression in HC samples. No significant correlation was found between snail and E-cadherin protein expression levels (P = 0.056). No significant correlation was found between snail protein expression levels and gender, age, tumor grade, vascular or perineural invasion, nodal metastasis and invasion, or proliferative index. Cancer samples with positive snail protein expression were associated with poor survival compared with the negative expresser groups. Kaplan-Meier curves comparing different snail protein expression levels to survival showed highly significant separation (P < 0.0001, log-rank test). With multivariate analysis, only snail protein expression among all parameters was found to influence survival (P = 0.0003). We suggest that snail expression levels can predict poor survival regardless of pathological features and tumor proliferation. Immunohistochemical detection of snail protein expression levels in routine sections may provide the first biological prognostic marker

Experimental Study on the Bending Resistance of Hollow Slab Beams Strengthened with Prestressed Steel Strand Polyurethane Cement Composite

In order to explore the toughening performance and failure mechanism of hollow slab beams strengthened with prestressed steel strand polyurethane cement composite, three test beams (L1–L3) were strengthened and one test beam (L0) was used as a comparison. The influence of different tensile stresses of steel strand and fiber additions on the flexural bearing capacity of the hollow slab beams, was studied. The cracking characteristics, load deflection relationship, ductility and strain of each test beam were compared and analyzed. The test results showed that the toughened material was well bonded to the hollow slab beam and the steel strand, which effectively inhibited the development of cracks in the test beams. The flexural bearing capacity of the strengthened test beams was significantly improved. The use of prestressed steel strand polyurethane cement composite material effectively improved the flexural bearing capacity of the test beams, and this reinforcement process can be further extended to engineering applications

Favorable Marker Alleles for Panicle Exsertion Length in Rice (Oryza sativa L.) Mined by Association Mapping and the RSTEP-LRT Method

The panicle exsertion length (PEL) in rice (Oryza sativa L.) is an important trait for hybrid seed production. We investigated the PEL in a chromosome segment substitution line (CSSL) population consisting of 66 lines and a natural population composed of 540 varieties. In the CSSL population, a total of seven QTLs for PEL were detected across two environments. The percentage of phenotypic variance explained (PVE) ranged from 10.22 to 50.18%, and the additive effect ranged from −1.77 to 6.47 cm. Among the seven QTLs, qPEL10.2 had the largest PVE, 44.05 and 50.18%, with an additive effect of 5.91 and 6.47 cm in 2015 and in 2016, respectively. In the natural population, 13 SSR marker loci were detected that were associated with PEL in all four environments, with the PVE ranging from 1.20 to 6.26%. Among the 13 loci, 7 were novel. The RM5746-170 bp allele had the largest phenotypic effect (5.11 cm), and the typical carrier variety was Qiaobinghuang. An RM5620-RM6100 region harboring the EUI2 locus on chromosome 10 was detected in both populations. The sequencing results showed that the accessions with a shorter PEL contained the A base, while the accessions with a longer PEL contained the G base at the 1,475 bp location of the EUI2 gene

A multiplex TaqMan real-time RT-PCR assay for the simultaneous detection of H4, H6, and H10 avian influenza viruses

Avian influenza viruses (AIVs) have caused a large number of epidemics in domestic and wild birds, and even posed a health challenge to humans. Highly pathogenic AIVs have attracted the most public attention. However, low pathogenic AIVs, including H4, H6, and H10 subtype AIVs, have spread covertly in domestic poultry, without obvious clinical signs. The emergence of human infections with H6 and H10 AIVs and the evidence of seropositivity of H4 AIV in poultry-exposed individuals indicated that these AIVs sporadically infect humans and could cause a potential pandemic. Therefore, a rapid and sensitive diagnostic method to simultaneously detect Eurasian lineage H4, H6, and H10 subtype AIVs is urgently required. Four singleplex real-time RT-PCR (RRT-PCR) assays were established based on carefully designed primers and probes of the conserved regions of the matrix, H4, H6, and H10 genes and combined into a multiplex RRT-PCR method to simultaneously detect H4, H6, and H10 AIVs in one reaction. The detection limit of the multiplex RRT-PCR method was 1–10 copies per reaction when detecting standard plasmids, and showed no cross-reaction against other subtype AIVs and other common avian viruses. Additionally, this method was suitable to detect the AIVs in samples from different sources, the results of which showed high consistency with virus isolation and a commercial influenza detection kit. In summary, this rapid, convenient, and practical multiplex RRT-PCR method could be applied in laboratory testing and clinical screening to detect AIVs

The effect of microbial inoculant origin on the rhizosphere bacterial community composition and plant growth-promotion

Microbial inoculation has been proposed as a potential approach for rhizosphere engineering. However, it is still unclear to what extent successful plant growth-promoting effects are driven by the origin of the microbial inocula and which taxa are responsible for the plant-beneficial effects

Image_2_Comparative transcriptomics reveal different genetic adaptations of biofilm formation in Bacillus subtilis isolate 1JN2 in response to Cd2+ treatment.jpg

Biofilm plays important roles in the life cycle of Bacillus species, such as promoting host and object surface colonization and resisting heavy metal stress. This study utilized transcriptomics to evaluate the impacts of cadmium on the components, morphology, and function of biofilms of Bacillus subtilis strain 1JN2. Under cadmium ion stress, the morphology of the B. subtilis 1JN2 biofilm was flattened, and its mobility increased. Moreover, differential gene expression analysis showed that the main regulator of biofilm formation, Spo0A, decreased in expression under cadmium ion stress, thereby inhibiting extracellular polysaccharide synthesis through the SinI/SinR two-component regulatory system and the AbrB pathway. Cadmium ion treatment also increased the SigD content significantly, thereby increasing the expression of the flagella encoding and assembly genes in the strain. This promoted poly-γ-glutamic acid production via the DegS/DegU two-component regulatory system and the conversion of biofilm extracellular polysaccharide to poly-γ-glutamic acid. This conferred cadmium stress tolerance in the strain. Additionally, the cadmium ion-mediated changes in the biofilm composition affected the colonization of the strain on the host plant root surface. Cadmium ions also induced surfactin synthesis. These findings illustrate the potential of Bacillus species as biocontrol strains that can mitigate plant pathogenic infections and heavy metal stress. The results also provide a basis for the screening of multifunctional biocontrol strains.</p